Intersite Structural Rearrangement of the Blue Copper Site Induced by Substrate Binding: Spectroscopic Studies of a Copper-Containing Nitrite Reductase from Alcaligenes xylosoxidans NCIM
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- 作者：Takamitsu Kohzuma ; Makiko Kikuchi ; Naoko Horikoshi ; Shigenori Nagatomo ; Teizo Kitagawa ; Roman S. Czernuszewicz
- 刊名：Inorganic Chemistry
- 出版年：2006
- 出版时间：October 16, 2006
- 年：2006
- 卷：45
- 期：21
- 页码：8474 - 8476
- 全文大小：81K
- 年卷期：v.45,no.21(October 16, 2006)
- ISSN：1520-510X
文摘
A copper-containing nitrite reductase from Alcaligenes xylosoxidansNCIMB 11015 has its own unique blue or type 1 copper proteinresonance Raman spectrum in the usual Cu-SCys stretching region,
(Cu-SCys), with a pair of strong peaks at 412 and 420 cm-1 anda weak peak at 364 cm-1. The predominantly (Cu-SCys) Ramanbands at 412, 420, and 364 cm-1 of the type 1 copper site allshifted to higher frequencies upon binding of nitrite to the type 2copper site, and the resonance Raman difference spectra progressively intensified with the increments of nitrite ion concentration.Positive support for substrate binding to the type 2 copper isprovided by the (Cu-SCys) bands in the resonance Ramanspectrum of a type 2 copper-depleted enzyme, which is insensitiveto the presence of NO2-. The shift to higher frequency of theRaman bands of the type 1 copper center with the addition ofnitrite ions suggests a stronger Cu-SCys interaction in the substrate-bound A. xylosoxidans nitrite reductase.
(Cu-SCys), with a pair of strong peaks at 412 and 420 cm-1 anda weak peak at 364 cm-1. The predominantly (Cu-SCys) Ramanbands at 412, 420, and 364 cm-1 of the type 1 copper site allshifted to higher frequencies upon binding of nitrite to the type 2copper site, and the resonance Raman difference spectra progressively intensified with the increments of nitrite ion concentration.Positive support for substrate binding to the type 2 copper isprovided by the (Cu-SCys) bands in the resonance Ramanspectrum of a type 2 copper-depleted enzyme, which is insensitiveto the presence of NO2-. The shift to higher frequency of theRaman bands of the type 1 copper center with the addition ofnitrite ions suggests a stronger Cu-SCys interaction in the substrate-bound A. xylosoxidans nitrite reductase.