High Conversion in Asymmetric Hydrolysis during Permeation through Enzyme-Multilayered Porous Hollow-Fiber Membranes

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• 作者：Tomomi Kawai ; Masanori Nakamura ; Kazuyuki Sugita ; Kyoichi Saito ; and Takanobu Sugo
• 刊名：Biotechnology Progress
• 出版年：2001
• 出版时间：October 2001
• 年：2001
• 卷：17
• 期：5
• 页码：872 - 875
• 全文大小：68K
• 年卷期：v.17,no.5(October 2001)
• ISSN：1520-6033

文摘

We describe a novel porous hollow-fiber support for immobilizing aminoacylase inmultilayers. Epoxy-group-containing polymer chains were grafted onto a porous hollow-fiber membrane by radiation-induced graft polymerization of glycidyl methacrylate,and subsequently a diethylamino group as an anion-exchange group was introducedinto the graft chain. Aminoacylase was adsorbed in multilayers by allowing theamioacylase buffer solution to permeate through the pores across the hollow fiber;the graft chains provided three-dimensional space for the enzymes because of theirelectrostatic repulsion. The adsorbed enzyme at a degree of multilayer binding of 15was cross-linked with glutaraldehyde to prevent leakage. An acetyl-DL-methioninesolution was allowed to permeate through the pores surrounded by the aminoacylase-immobilized graft chain. Production of L-methionine was observed at a 4.1 mol/h perL of the fiber for a space velocity of 200 h^-1, defined as the flow rate of the effluentpenetrating the outside surface of the hollow fiber divided by the membrane volumeincluding the lumen.