Purification of Docosahexaenoic Acid Ethyl Ester Using a Silver-Ion-Immobilized Porous Hollow-Fiber Membrane Module

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• 作者：Ichiro Ozawa ; Min Kim ; Kyoichi Saito ; Kazuyuki Sugita ; Takashi Baba ; Shigeru Moriyama ; and Takanobu Sugo
• 刊名：Biotechnology Progress
• 出版年：2001
• 出版时间：October 2001
• 年：2001
• 卷：17
• 期：5
• 页码：893 - 896
• 全文大小：82K
• 年卷期：v.17,no.5(October 2001)
• ISSN：1520-6033

文摘

Docosahexaenoic acid ethyl ester (DHA-Et) was purified by adsorption on Ag-ion-immobilized membranes via selective interaction between silver ion and carbon-carbondouble bonds of DHA-Et. Silver ions were immobilized onto sulfonic-acid-group-containing porous hollow-fiber membranes at an Ag ion density of 1.4 mol/kg ofmembrane, and 30 membranes were housed in one module (inner diameter = 18 mmand effective length = 80 mm). The adsorption isotherms of DHA-Et in various organicsolvents revealed that DHA-Et was adsorbed on the immobilized Ag ions with a DHA-Et/Ag ion molar binding ratio of 1/5 in methanol, and that acetonitrile was the solventof choice for the elution of the adsorbed DHA-Et. Permeation of bonito oil ethyl estersolution in methanol through the Ag-ion-immobilized hollow-fiber membrane moduledemonstrated that the displacement adsorption of other lower unsaturated fatty-acidethyl esters by DHA-Et proceeded along the membrane thickness. The purity of DHA-Et was improved to 99 wt % by permeating first bonito oil ethyl ester containing 95wt % DHA-Et and then acetonitrile through the module.