Production of Cycloisomaltooligosaccharides from Dextran Using Enzyme Immobilized in Multilayers onto Porous Membranes
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- 作者：Hidetaka Kawakita ; Kazuyuki Sugita ; Kyoichi Saito ; Masao Tamada ; Takanobu Sugo ; and Hiroshi Kawamoto
- 刊名：Biotechnology Progress
- 出版年：2002
- 出版时间：June 2002
- 年：2002
- 卷：18
- 期：3
- 页码：465 - 469
- 全文大小：97K
- 年卷期：v.18,no.3(June 2002)
- ISSN：1520-6033
文摘
Anion-exchange porous hollow-fiber membranes with a thickness of about 1.2 mmand a pore size of about 0.30 
m were used as a supporting matrix to immobilizecycloisomaltooligosaccharide glucanotransferase (CITase). CITase was immobilized tothe membrane via anion-exchange adsorption and by subsequent enzymatic cross-linking with transglutaminase, the amount of which ranged from 3 to 110 mg pergram of the membrane. The degree of enzyme multilayer binding was equivalent to0.3-9.8. Dextran, as the substrate, was converted into seven- to nine-glucose-membered cycloisomaltooligosaccharides (CI-7, -8, and -9) at a maximum yield of 28%in weight at a space velocity of 10 per hour during the permeation of 2.0% (w/w) dextransolution across the CITase-immobilized porous hollow-fiber membrane. The yield ofCIs increased with increasing degree of CITase multilayering.
m were used as a supporting matrix to immobilizecycloisomaltooligosaccharide glucanotransferase (CITase). CITase was immobilized tothe membrane via anion-exchange adsorption and by subsequent enzymatic cross-linking with transglutaminase, the amount of which ranged from 3 to 110 mg pergram of the membrane. The degree of enzyme multilayer binding was equivalent to0.3-9.8. Dextran, as the substrate, was converted into seven- to nine-glucose-membered cycloisomaltooligosaccharides (CI-7, -8, and -9) at a maximum yield of 28%in weight at a space velocity of 10 per hour during the permeation of 2.0% (w/w) dextransolution across the CITase-immobilized porous hollow-fiber membrane. The yield ofCIs increased with increasing degree of CITase multilayering.