Near-Infrared Light-Directed RNAi Using a Photosensitive Carrier Molecule

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• 作者：Yuka Matsushita-Ishiodori ; Mika Morinaga ; Kazunori Watanabe ; Takashi Ohtsuki
• 刊名：Bioconjugate Chemistry
• 出版年：2013
• 出版时间：October 16, 2013
• 年：2013
• 卷：24
• 期：10
• 页码：1669-1673
• 全文大小：297K
• 年卷期：v.24,no.10(October 16, 2013)
• ISSN：1520-4812

文摘

Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.