Multistep Growth Mechanism of Calcium Phosphate in the Earliest Stage of Morphology-Controlled Biomineralization

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• 作者：Takayuki Nonoyama ; Takatoshi Kinoshita ; Masahiro Higuchi ; Kenji Nagata ; Masayoshi Tanaka ; Kimiyasu Sato ; Katsuya Kato
• 刊名：Langmuir
• 出版年：2011
• 出版时间：June 7, 2011
• 年：2011
• 卷：27
• 期：11
• 页码：7077-7083
• 全文大小：976K
• 年卷期：v.27,no.11(June 7, 2011)
• ISSN：1520-5827

文摘

We studied the effect of surface-functional-group position on precipitate morphology in the earliest stage of calcium phosphate biomineralization and determined the detailed mechanism of precipitation starting from nucleation to precipitate growth. The biomineralization template was a 尾-sheet peptide scaffold prepared by adsorption with carboxyl groups arranged at strict 7 脜 intervals. Phosphate was then introduced. Within 10 s, highly ordered embryos of calcium phosphate were formed and confined by a peptide nanofiber pattern. They repeatedly nucleated and dissolved, with the larger embryos absorbing the smaller ones in a clear demonstration of an Ostwald-ripening-like phenomenon, then aggregated in a line pattern, and finally formed highly ordered nanofibers of amorphous calcium phosphate. This multistep growth process constitutes the earliest stage of biomineralization.