Cell Sorting Technique Based on Thermoresponsive Differential Cell Adhesiveness
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- 作者：Takehisa Matsuda ; Yuko Saito ; Kaori Shoda
- 刊名：Biomacromolecules
- 出版年：2007
- 出版时间：August 2007
- 年：2007
- 卷：8
- 期：8
- 页码：2345 - 2349
- 全文大小：164K
- 年卷期：v.8,no.8(August 2007)
- ISSN：1526-4602
文摘
Cell sorting of specific target cells from a mixture of different cell types is a prerequisite for development offunctional engineered tissues based on stem-cell and tissue engineering. This paper presents a new method of cellsorting that uses a mixture of thermoresponsive cell-adhesive and non-cell-adhesive substances. The formersubstance is poly(N-isopropylacrylamide)-grafted gelatin (PNIPAM-gelatin) and the latter is PNIPAM. Gradedcell adhesion, produced by mixed coating of these thermoresponsive substances at an appropriate mixing ratio,clearly differentiated the adhesive potentials of two bovine vascular cell types (endothelial cell and smooth musclecell). The sequential procedures of detachment at room temperature and subsequent replating at 37 
C on dishescoated with a mixed coating with the same composition as that employed previously yielded remarkably puretarget cells, as determined using confocal laser scanning fluorescence microscopy. This method, leading to harvestingof target cells, is characteristic of simple manipulation with no cell damage. Such advantages are expected tofacilitate stem-cell and tissue engineering.
C on dishescoated with a mixed coating with the same composition as that employed previously yielded remarkably puretarget cells, as determined using confocal laser scanning fluorescence microscopy. This method, leading to harvestingof target cells, is characteristic of simple manipulation with no cell damage. Such advantages are expected tofacilitate stem-cell and tissue engineering.