Formation of Polymerized Mixed Heparin/Albumin Surface Layer and Cellular Adhesional Responses

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• 作者：Tomoko Magoshi and Takehisa Matsuda
• 刊名：Biomacromolecules
• 出版年：2002
• 出版时间：September 2002
• 年：2002
• 卷：3
• 期：5
• 页码：976 - 983
• 全文大小：403K
• 年卷期：v.3,no.5(September 2002)
• ISSN：1526-4602

文摘

The aim of this study was to create a dense albuminated layer, a heparinized layer, and a mixed layer ona poly(acrylic acid)-grafted surface via visible light induced photopolymerization. The procedure is comprisedof four reaction steps: first, by visible light irradiation, acrylic acid (AA) was graft-polymerized on asegmented polyurethane (SPU) film that was preimpregnated with camphorquinone. The second step wasadsorption of multiply styrenated albumin or styrenated heparin or their mixture, followed by visible lightirradiation in the presence of carboxylated camphorquinone. The third step was covalent bonding betweenpolyAA graft chain and polymerized biomacromolecule and between polymerized biomacromolecule toenforce the formation of a stable immobilized multilayer. X-ray photoelectron spectroscopic and Fouriertransform-infrared spectroscopic measurements were conducted to analyze the surfaces formed at each step.Confocal laser scanning microscopy was utilized to determine the thickness of the biomacromolecule-immobilized layer with several tenths of a micrometer thickness. Platelet adhesion was markedly reducedon polymerized albuminated, polymerized heparinized, and copolymerized layers, whereas adhesive andproliferative potentials of endothelial cells, which were comparable to those of commercial tissue culturedishes, were observed on these surfaces. Co-immobilization of fibronectin and basic fibroblast growth factorenhanced these potentials. These densely multilayered surfaces may be suitable for artificial and tissue-engineered devices.