Co-Electrospun Nanofiber Fabrics of Poly(L-lactide-co--caprolactone) with Type I Collagen or Heparin
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- 作者：Il Keun Kwon and Takehisa Matsuda
- 刊名：Biomacromolecules
- 出版年：2005
- 出版时间：July 2005
- 年：2005
- 卷：6
- 期：4
- 页码：2096 - 2105
- 全文大小：1259K
- 年卷期：v.6,no.4(July 2005)
- ISSN：1526-4602
文摘
Functionally designed elastomeric nanofiber fabrics made of the equimolar copolyester, poly(L-lactide-co-
-caprolactone) (PLCL), with type I collagen or the tri-n-butylamine salt of heparin (heparin-TBA) wereco-electrospun using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) as a solvent. The co-electrospun fabrics (mixingratio: 0, 5, 10, 30, 50, 70, and 100 wt % of collagen to PLCL) consisted of nanoscale fibers with a meandiameter ranging from approximately 120 to 520 nm. An increase in collagen content in the solution resultedin a decrease in the mean diameter of fibers. Transmission electron microscopy (TEM) showed that collagenin a co-electrospun fiber was phase-separated to form a dispersed phase, which was localized in the interiorand peripheral region in the continuous matrix phase of fibers. The tensile strength was decreased withincreasing collagen content. Human umbilical vein endothelial cells (HUVECs) were highly elongated andwell spread on the fibrous surfaces of fabrics made of PLCL with 5 wt % or 10 wt % collagen. Heparin-TBA (mixing ratio: 1, 5, and 10 wt % to PLCL), soluble in HFIP, was co-electrospun with PLCL to forma fabric. TEM observation showed that heparin-TBA formed as a dispersed phase in a PLCL nanofiber.The releasing rate, released amount, and surface content of heparin-TBA were increased with increasingheparin-TBA content in co-electrospun fabrics. The potential biomedical application of co-electrospun PLCLwith type I collagen or heparin-TBA was discussed.
-caprolactone) (PLCL), with type I collagen or the tri-n-butylamine salt of heparin (heparin-TBA) wereco-electrospun using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) as a solvent. The co-electrospun fabrics (mixingratio: 0, 5, 10, 30, 50, 70, and 100 wt % of collagen to PLCL) consisted of nanoscale fibers with a meandiameter ranging from approximately 120 to 520 nm. An increase in collagen content in the solution resultedin a decrease in the mean diameter of fibers. Transmission electron microscopy (TEM) showed that collagenin a co-electrospun fiber was phase-separated to form a dispersed phase, which was localized in the interiorand peripheral region in the continuous matrix phase of fibers. The tensile strength was decreased withincreasing collagen content. Human umbilical vein endothelial cells (HUVECs) were highly elongated andwell spread on the fibrous surfaces of fabrics made of PLCL with 5 wt % or 10 wt % collagen. Heparin-TBA (mixing ratio: 1, 5, and 10 wt % to PLCL), soluble in HFIP, was co-electrospun with PLCL to forma fabric. TEM observation showed that heparin-TBA formed as a dispersed phase in a PLCL nanofiber.The releasing rate, released amount, and surface content of heparin-TBA were increased with increasingheparin-TBA content in co-electrospun fabrics. The potential biomedical application of co-electrospun PLCLwith type I collagen or heparin-TBA was discussed.