Integration of an Immunosorbent Assay System: Analysis of Secretory Human Immunoglobulin A on Polystyrene Beads in a Microchip

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• 作者：Kiichi Sato ; Manabu Tokeshi ; Tamao Odake ; Hiroko Kimura ; Takeshi Ooi ; Masayuki Nakao ; and Takehiko Kitamori
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：March 15, 2000
• 年：2000
• 卷：72
• 期：6
• 页码：1144 - 1147
• 全文大小：153K
• 年卷期：v.72,no.6(March 15, 2000)
• ISSN：1520-6882

文摘

An immunosorbent assay system was integrated into aglass microchip. Polystyrene beads were introduced intoa microchannel, and then human secretory immunoglobulin A (s-IgA) adsorbed on the bead surface was reactedwith colloidal gold conjugated anti-s-IgA antibody anddetected by a thermal lens microscope. The scale meritsof liquid microspace on the molecular behavior remarkably contributed to reduced assay time. The integrationcut the time necessary for the antigen-antibody reactionby ¹/₉₀, thus shortening the overall analysis time from 24h to less than 1 h. Moreover, troublesome operationsrequired for conventional immunosorbent assays couldbe replaced by simple operations.