Structural Evaluation of Tandem Hairpin Pyrrole鈥揑midazole Polyamides Recognizing Human Telomeres

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• 作者：Akiyoshi Hirata ; Kiyoshi Nokihara ; Yusuke Kawamoto ; Toshikazu Bando ; Asuka Sasaki ; Satoru Ide ; Kazuhiro Maeshima ; Takeshi Kasama ; Hiroshi Sugiyama
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：August 13, 2014
• 年：2014
• 卷：136
• 期：32
• 页码：11546-11554
• 全文大小：571K
• ISSN：1520-5126

文摘

A polyamide containing N-methylpyrrole (Py) and N-methylimidazole (Im), designated PIPA, binds with high affinity and specificity to specific nucleotide sequences in the minor groove of double-helical DNA. Based on a recent report of the synthesis of PIPA for telomere visualization, the present paper focused on the size of the connecting part (hinge region) of two PIPA segments of the tandem hairpin PIPA, Dab(Im-Im-Py)-Py-Py-Py-Im-[Hinge]-Dab(Im-Im-Py)-Py-Py-Py-Im-尾Ala-NH(CH₂)₃N(CH₃)-(CH₂)₃NH-[Dye]. The present paper also describes the characterization of binding by measuring the thermal melting temperature and surface plasmon resonance and by specific staining of telomeres (TTAGGG)n in human cells. Microheterogeneity was also investigated by high-resolution mass spectrometry. We found that the optimal compound as the hinge segment for telomere staining was [-NH(C₂H₄O)₂(C₂H₄)CO-] with tetramethylrhodamine as the fluorescent dye.