Metabolic Profiling of Transgenic Potato Tubers Expressing Arabidopsis Dehydration Response Element-Binding Protein 1A (DREB1A)
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- 作者：Toshio Iwaki ; Lining Guo ; John A. Ryals ; Syuhei Yasuda ; Takayoshi Shimazaki ; Akira Kikuchi ; Kazuo N. Watanabe ; Mie Kasuga ; Kazuko Yamaguchi-Shinozaki ; Takumi Ogawa ; Daisaku Ohta
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：January 30, 2013
- 年：2013
- 卷：61
- 期：4
- 页码：893-900
- 全文大小：375K
- 年卷期：v.61,no.4(January 30, 2013)
- ISSN：1520-5118
文摘
Untargeted metabolome analyses play a critical role in understanding possible metabolic fluctuations of crops under varying environmental conditions. This study reports metabolic profiles of transgenic potato tubers expressing the ArabidopsisDREB1A transcription factor gene, which induces expression of genes involved in environmental stress tolerance. A combination of targeted and untargeted metabolomics demonstrated considerable metabolome differences between the transgenic lines and nontransgenic parent cultivars. In the transgenic lines, stimulation of stress responses was suggested by elevated levels of the glutathione metabolite, 纬-aminobutyric acid (GABA), and by the accumulation of 尾-cyanoalanine, a byproduct of ethylene biosynthesis. These results suggest that the ArabidopsisDREB1A expression might directly or indirectly enhance endogenous potato stress tolerance systems. The results indicate that transgenesis events could alter the metabolic compositions in food crops, and therefore metabolomics analysis could be a most valuable tool to monitor such changes.