Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

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• 作者：Takuya Terai ; Moe Kohno ; Gaelle Boncompain ; Shigeru Sugiyama ; Nae Saito ; Ryo Fujikake ; Tasuku Ueno ; Toru Komatsu ; Kenjiro Hanaoka ; Takayoshi Okabe ; Yasuteru Urano ; Franck Perez ; Tetsuo Nagano
• 刊名：Journal of the American Chemical Society
• 出版年：2015
• 出版时间：August 26, 2015
• 年：2015
• 卷：137
• 期：33
• 页码：10464-10467
• 全文大小：367K
• ISSN：1520-5126

文摘

Artificial ligands of streptavidin (ALiS) with association constants of 鈭?0⁶ M^鈥? were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.