Response of Cells on Surface-Induced Nanopatterns: Fibroblasts and Mesenchymal Progenitor Cells

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• 作者：Hwei Ling Khor ; Yujun Kuan ; Hildegard Kukula ; Kaoru Tamada ; Wolfgang Knoll ; Martin Moeller ; Dietmar W. Hutmacher ; &
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：May 2007
• 年：2007
• 卷：8
• 期：5
• 页码：1530 - 1540
• 全文大小：2669K
• 年卷期：v.8,no.5(May 2007)
• ISSN：1526-4602

文摘

Ultrathin films of a poly(styrene)-block-poly(2-vinylpyrindine) diblock copolymer (PS-b-P2VP) and poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer (PS-b-P4VP) were used to form surface-induced nanopattern(SINPAT) on mica. Surface interaction controlled microphase separation led to the formation of chemicallyheterogeneous surface nanopatterns on dry ultrathin films. Two distinct nanopatterned surfaces, namely, wormlikeand dotlike patterns, were used to investigate the influence of topography in the nanometer range on cell adhesion,proliferation, and migration. Atomic force microscopy was used to confirm that SINPAT was stable under cellculture conditions. Fibroblasts and mesenchymal progenitor cells were cultured on the nanopatterned surfaces.Phase contrast and confocal laser microscopy showed that fibroblasts and mesenchymal progenitor cells preferredthe densely spaced wormlike patterns. Atomic force microscopy showed that the cells remodelled the extracellularmatrix differently as they migrate over the two distinctly different nanopatterns.