Enhancement of Surface Plasmon Resonance Signals by Gold Nanoparticles on High-Density DNA Microarrays
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- 作者：Masateru Ito ; Fumio Nakamura ; Akira Baba ; Kaoru Tamada ; Hirobumi Ushijima ; King Hang Aaron Lau ; Abhijit Manna ; Wolfgang Knoll
- 刊名：Journal of Physical Chemistry C
- 出版年：2007
- 出版时间：August 9, 2007
- 年：2007
- 卷：111
- 期：31
- 页码：11653 - 11662
- 全文大小：3211K
- 年卷期：v.111,no.31(August 9, 2007)
- ISSN：1932-7455
文摘
We have developed a new methodology to fabricate high-resolution DNA microarrays (2500 dots/cm2) incombination with microcontact printing (
CP) and surface plasmon resonance (SPR) imaging. A novel COOH-terminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically boundto a gold substrate for surface coupling reactions with NH2-terminated DNA. The hybridization betweentarget DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfullydemonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. Thegold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles(~10%) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancementeffect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectricconstants and Fresnel's equation.
CP) and surface plasmon resonance (SPR) imaging. A novel COOH-terminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically boundto a gold substrate for surface coupling reactions with NH2-terminated DNA. The hybridization betweentarget DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfullydemonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. Thegold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles(~10%) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancementeffect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectricconstants and Fresnel's equation.