Dynamic Interfacial Properties of Poly(ethylene glycol)-Modified Ferritin at the Solid/Liquid Interface

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• 作者：Yoshikazu Kumashiro ; Yasuhiro Ikezoe ; Kaoru Tamada ; Masahiko Hara
• 刊名：Journal of Physical Chemistry B
• 出版年：2008
• 出版时间：July 17, 2008
• 年：2008
• 卷：112
• 期：28
• 页码：8291 - 8297
• 全文大小：1036K
• 年卷期：v.112,no.28(July 17, 2008)
• ISSN：1520-5207

文摘

Poly(ethylene glycol)-modified ferritins (PEG-ferritins) with various molecular weights were synthesized by the grafting method, and their dynamic interfacial properties at the solid/liquid interface were investigated. The number of PEG grafted to ferritins was controlled by the amount of 1,1′-carbonyldiimidazole-modified PEG adding to the reaction solution. The adsorption kinetics and energy dissipation of PEG-ferritins onto bare Si substrate and amino-modified Si substrate were investigated with a quartz crystal microbalance (QCM) in 10 mM bis-Tris/HCl buffer (pH 5.8), while their morphologies were characterized by scanning electron microscopy (SEM). The adsorption dynamics of PEG-ferritins onto amino-modified Si substrate were quite different from those of unmodified ferritin, which can be reasonably interpreted by the desorption capability of PEG-ferritins on the surface attributed to amphiphilicity and the high-chain mobility of PEG chains.