Photosensitizing Carrier Proteins for Photoinducible RNA Interference

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• 作者：Yuka Matsushita-Ishiodori ; Rina Kuwabara ; Hiroyuki Sakakoshi ; Tamaki Endoh ; Takashi Ohtsuki
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：November 16, 2011
• 年：2011
• 卷：22
• 期：11
• 页码：2222-2226
• 全文大小：353K
• 年卷期：v.22,no.11(November 16, 2011)
• ISSN：1520-4812

文摘

RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.