Unusual 鈭? Ribosomal Frameshift Caused by Stable RNA G-Quadruplex in Open Reading Frame

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• 作者：Tamaki Endoh ; Naoki Sugimoto
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：December 3, 2013
• 年：2013
• 卷：85
• 期：23
• 页码：11435-11439
• 全文大小：316K
• 年卷期：v.85,no.23(December 3, 2013)
• ISSN：1520-6882

文摘

Tertiary structures formed by mRNAs impact the efficiency of the translation reaction. Ribosomal frameshift is a well-characterized recoding process that occurs during translation elongation. Pseudoknot and stem-loop structures may stimulate frameshifting by causing a translational halt at a slippery sequence. In this study, we evaluated the efficiency of an unusual 鈭? frameshift caused by a noncanonical RNA G-quadruplex structure in mammalian cells. The reporter gene construct consisting of a fluorescent protein and Luciferase enabled evaluation of apparent and absolute values of the 鈭? frameshift efficiency and revealed significant increase of the efficiency by G-quadrupex forming potential sequence. In addition, berberine, a small molecule that binds to and stabilizes G-quadruplex structures, further increased the frameshift efficiency. These results indicate that the stable G-quadruplex structure stimulates the unusual 鈭? frameshift and has a potential to regulate the frameshift with its ligand.