Translation of in Vitro Inhibition by Marine Natural Products of the C4 Acid Cycle Enzyme Pyruvate Pi Dikinase to in Vivo C4 Plant Tissue Death

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• 作者：Dianne S. Haines ; James N. Burnell ; Jason R. Doyle ; Lyndon E. Llewellyn ; Cherie A. Motti ; and Dianne M. Tapiolas
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：May 18, 2005
• 年：2005
• 卷：53
• 期：10
• 页码：3856 - 3862
• 全文大小：460K
• 年卷期：v.53,no.10(May 18, 2005)
• ISSN：1520-5118

文摘

Marine organism derived extracts, previously identified as containing compounds that inhibited theC₄ acid cycle enzyme pyruvate P_i dikinase (PPDK), were assessed for their ability to exhibit an effecton the C₄ plants Digitaria ciliaris and Echinochloa crus-galli. Oxygen electrode studies revealed thatover half of these extracts inhibited C₄ acid driven photosynthesis in leaf slices. Seventeen extractshad a deleterious effect on C₄ plants in vivo within 24 h, whereas 36 caused an observable phytotoxicresponse in one or both of the C₄ plants used for in vivo testing. None of the extracts inhibited PPDKmetabolism of pyruvate via a directly competitive mechanism, instead hindering the enzyme by eithermixed or uncompetitive means. This screening strategy, using a suite of assays, led to the isolationand identification of the herbicidal marine natural product ilimaquinone.Keywords: Herbicide; PPDK; C₄ plant; oxygen evolution