Kiwifruit Flower Odor Perception and Recognition by Honey Bees, Apis mellifera

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• 作者：Andrew M. Twidle ; Flore Mas ; Aimee R. Harper ; Rachael M. Horner ; Taylor J. Welsh ; David M. Suckling
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：June 17, 2015
• 年：2015
• 卷：63
• 期：23
• 页码：5597-5602
• 全文大小：230K
• ISSN：1520-5118

文摘

Volatile organic compounds (VOCs) from male and female kiwifruit (Actinidia deliciosa 鈥楬ayward鈥? flowers were collected by dynamic headspace sampling. Honey bee (Apis mellifera) perception of the flower VOCs was tested using gas chromatography coupled to electroantennogram detection. Honey bees consistently responded to six compounds present in the headspace of female kiwifruit flowers and five compounds in the headspace of male flowers. Analysis of the floral volatiles by gas chromatography鈥搈ass spectrometry and microscale chemical derivatization showed the compounds to be nonanal, 2-phenylethanol, 4-oxoisophorone, (3E,6E)-伪-farnesene, (6Z,9Z)-heptadecadiene, and (8Z)-heptadecene. Bees were then trained via olfactory conditioning of the proboscis extension response (PER) to synthetic mixtures of these compounds using the ratios present in each flower type. Honey bees trained to the synthetic mixtures showed a high response to the natural floral extracts, indicating that these may be the key compounds for honey bee perception of kiwifruit flower odor.