Echinacea has been known for its immunostimulatory activity, and its alkylamide components havebeen linked to such biological activity. Consequently, alkylamides in
Echinacea angustifolia wereextracted using supercritical carbon dioxide from fresh and dried roots at 45-60
C and 34-55 MPa,and the alkylamide yield in the extracts was determined. The yield of alkylamides from fresh rootsincreased with temperature yet decreased with pressure, whereas the yield from air-dried roots(moisture content 8.4%) increased with both temperature and pressure. Freeze-drying of the rootsto a moisture content of 4.9% did not result in any further increase in the yield compared to that ofair-dried roots. Alkylamide yield of the ground dried roots extract was the highest (
p 0.05) amongthose from fresh, ground and unground
E. angustifolia roots. Supercritical fluid extraction thereforeshows potential for the recovery of alkylamides from dried
Echinacea roots.Keywords: Alkylamide;
Echinacea angustifolia; supercritical fluid extraction