Speciation Analysis of Gadolinium-Based MRI Contrast Agents in Blood Plasma by Hydrophilic Interaction Chromatography/Electrospray Mass Spectrometry

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• 作者：Jens Kü ; nnemeyer ; Lydia Terborg ; Sascha Nowak ; Andy Scheffer ; Lena Telgmann ; Faruk Tokmak ; Andreas Gü ; nsel ; Gerhard Wiesmü ; ller ; Stephan Reichelt ; Uwe Karst
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：November 1, 2008
• 年：2008
• 卷：80
• 期：21
• 页码：8163-8170
• 全文大小：245K
• 年卷期：v.80,no.21(November 1, 2008)
• ISSN：1520-6882

文摘

The first analytical method for simultaneous speciation analysis of five of the most important gadolinium-based magnetic resonance imaging (MRI) contrast agents in blood plasma samples was developed. Gd-DTPA (Magnevist), Gd-BT-DO3A (Gadovist), Gd-DOTA (Dotarem), Gd-DTPA-BMA (Omniscan), and Gd-BOPTA (Multihance) were separated by hydrophilic interaction liquid chromatography (HILIC) and detected with electrospray mass spectrometry (ESI-MS). Spiking experiments of blank plasma with Magnevist and Gadovist were performed to determine the analytical figures of merit and the recovery rates. The limits of detection ranged from 1 × 10⁻⁷ to 1 × 10⁻⁶ mol/L depending on the ionization properties of the individual compounds, and limits of quantification ranged from 5 × 10⁻⁷ to 5 × 10⁻⁶ mol/L. The linear concentration range comprised 2 orders of magnitude. With application of this method, blood plasma samples of 10 healthy volunteers, with Magnevist or Gadovist medication, were analyzed for Gd-DTPA and Gd-BT-DO3A, respectively. The obtained results were successfully validated with inductively coupled plasma-optical emission spectroscopy (ICP-OES).