Modulating the Actions of NK Cell-Mediated Cytotoxicity Using Lipid-PEG (n) and Inhibitory Receptor-Specific Antagonistic Peptide Conjugates

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• 作者：Hyeyoung A. Chung ; Kyoko Tajima ; Koichi Kato ; Naoki Matsumoto ; Kazuo Yamamoto ; and Teruyuki Nagamune
• 刊名：Biotechnology Progress
• 出版年：2005
• 出版时间：August 2005
• 年：2005
• 卷：21
• 期：4
• 页码：1226 - 1230
• 全文大小：208K
• 年卷期：v.21,no.4(August 2005)
• ISSN：1520-6033

文摘

Regulating the cell surface modulates the actions of the biological cell response, derivespractical applications, and is of scientific interest. On the basis of our previous studyusing dioleylphosphatidylethanolamine poly(ethylene glycol) with multiple units ofethyleneoxide (DOPE-PEG (n)), we demonstrated the potency of DOPE-PEG (80) as acell surface modulator. We prepared conjugates of DOPE-PEG (80) and two antagonistic peptides (C1, SGGGCLFNLPWLCG; C26, SGGGCPFSFLPWCG), specificallydesigned for the inhibitory receptor of natural killer (NK) cells. We confirmed thatNK cells exhibited cytotoxicity against DOPE-PEG (80)-peptides-incorporated targetcells. We further investigated whether the DOPE-PEG (80)-peptides could affect thecytotoxicity of NK cells in a concentration-dependent manner. C1 peptide showed down-regulation of cytotoxicity at higher concentration, whereas C26 peptide exhibited thesaturated cytotoxicity of NK cells at the same concentration. These results suggestthat DOPE-PEG (80) can achieve the role of a cell surface modulator without inhibitingthe action of conjugated molecules, despite their relatively small size.