文摘
Covalently modified albumin (BSA) microparticles were developed for potential use as an adjuvant in mucosal vaccines against hepatitis B. To synthesize consistent protein particles, a covalent approach was proposed to modify BSA. Our strategy was to bond maleic anhydride (MA) molecules to BSA structure by nucleophilic reaction for further radical cross-linking/polymerization reaction with N鈥?N鈥?i>-dimethylacrylamide (DMAAm). The presence of poly(N鈥?N鈥?i>-dimethylacrylamide) in the protein network enables the microparticles to show well-defined, homogeneous forms. Cytotoxicity tests showed that the cytotoxic concentration for 50% of VERO cells (CC<sub>50sub>) was 216.25 卤 5.30 渭g mL<sup>鈥?sup> in 72 h of incubation. The obtained CC<sub>50sub> value is relatively low for an incubation time of 72 h, suggesting an acceptable biocompatibility. Assay of total protein showed that the encapsulation efficiency of the microparticles with hepatitis B surface antigen (HBsAg) was 77.7 卤 0.2%. For the reference sample, which was incubated without HBsAg, the quantity of protein was below the limit of detection.