Multimeric Near IR鈥揗R Contrast Agent for Multimodal In Vivo Imaging

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• 作者：Victoria S. R. Harrison ; Christiane E. Carney ; Keith W. MacRenaris ; Emily A. Waters ; Thomas J. Meade
• 刊名：Journal of the American Chemical Society
• 出版年：2015
• 出版时间：July 22, 2015
• 年：2015
• 卷：137
• 期：28
• 页码：9108-9116
• 全文大小：523K
• ISSN：1520-5126

文摘

Multiple imaging modalities are often required for in vivo imaging applications that require both high probe sensitivity and excellent spatial and temporal resolution. In particular, MR and optical imaging are an attractive combination that can be used to determine both molecular and anatomical information. Herein, we describe the synthesis and in vivo testing of two multimeric NIR鈥揗R contrast agents that contain three Gd(III) chelates and an IR-783 dye moiety. One agent contains a PEG linker and the other a short alkyl linker. These agents label cells with extraordinary efficacy and can be detected in vivo using both imaging modalities. Biodistribution of the PEGylated agent shows observable fluorescence in xenograft MCF7 tumors and renal clearance by MR imaging.