Improved Method for Quantifying the Avicide 3-Chloro-p-toluidine Hydrochloride in Bird Tissues Using a Deuterated Surrogate/GC/MS Method
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- 作者：Randal S. Stahl ; Thomas W. Custer ; Patricia A. Pochop ; and John J. Johnston
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2002
- 出版时间：February 13, 2002
- 年：2002
- 卷：50
- 期：4
- 页码：732 - 738
- 全文大小：79K
- 年卷期：v.50,no.4(February 13, 2002)
- ISSN：1520-5118
文摘
A method using a deuterated surrogate of the avicide 3-chloro-p-toluidine hydrochloride (CPTH) wasdeveloped to quantify the CPTH residues in the gastrointestinal (GI) tract and breast muscle tissuesin birds collected in CPTH-baited sunflower and rice fields. This method increased the range of aprevious surrogate/gas chromatography/mass spectroscopy method from 0-2 to 0-20 
g/g in tissuesamples and greatly simplified the extraction procedure. The modified method also sought to increaserecoveries over a range of matrix effects introduced by analyzing tissues from birds collected in thefield, where the GI tract contents would be affected by varying diet. The new method was used todetermine the CPTH concentration in GI tract samples fortified with CPTH-treated rice bait to simulatethe consumption of varying amounts of treated bait by two nontargeted bird species, pigeon (Columbialivia) and house sparrow (Passer domesticus). The new method was then used to examine the CPTHconcentrations in the gizzard contents of the targeted bird species, red-winged black bird (Agelaiusphoeniceus) and brown-headed cowbird (Molothrus ater), that were collected after feeding at a treatedbait site. The method proved sufficiently sensitive to quantify CPTH in the breast muscle tissues andthe gizzard contents of red-winged blackbirds and brown-headed cowbirds during an operationalbaiting program. The levels of CPTH determined for these birds in both tissue samples weredetermined to be highly correlated. The appearance of CPTH in the breast muscle tissue immediatelyafter feeding was not anticipated. The potential secondary hazard posed by the targeted birds topotential scavengers and predators was also evaluated.Keywords: 3-Chloro-p-toluidine hydrochloride; CPTH; DRC-1339; secondary hazard
g/g in tissuesamples and greatly simplified the extraction procedure. The modified method also sought to increaserecoveries over a range of matrix effects introduced by analyzing tissues from birds collected in thefield, where the GI tract contents would be affected by varying diet. The new method was used todetermine the CPTH concentration in GI tract samples fortified with CPTH-treated rice bait to simulatethe consumption of varying amounts of treated bait by two nontargeted bird species, pigeon (Columbialivia) and house sparrow (Passer domesticus). The new method was then used to examine the CPTHconcentrations in the gizzard contents of the targeted bird species, red-winged black bird (Agelaiusphoeniceus) and brown-headed cowbird (Molothrus ater), that were collected after feeding at a treatedbait site. The method proved sufficiently sensitive to quantify CPTH in the breast muscle tissues andthe gizzard contents of red-winged blackbirds and brown-headed cowbirds during an operationalbaiting program. The levels of CPTH determined for these birds in both tissue samples weredetermined to be highly correlated. The appearance of CPTH in the breast muscle tissue immediatelyafter feeding was not anticipated. The potential secondary hazard posed by the targeted birds topotential scavengers and predators was also evaluated.Keywords: 3-Chloro-p-toluidine hydrochloride; CPTH; DRC-1339; secondary hazard