CARM1 Preferentially Methylates H3R17 over H3R26 through a Random Kinetic Mechanism

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• 作者：Suzanne L. Jacques ; Katrina P. Aquino ; Jodi Gureasko ; P. Ann Boriack-Sjodin ; Margaret Porter Scott ; Robert A. Copeland ; Thomas V. Riera
• 刊名：Biochemistry
• 出版年：2016
• 出版时间：March 22, 2016
• 年：2016
• 卷：55
• 期：11
• 页码：1635-1644
• 全文大小：476K
• ISSN：1520-4995

文摘

CARM1 is a type I arginine methyltransferase involved in the regulation of transcription, pre-mRNA splicing, cell cycle progression, and the DNA damage response. CARM1 overexpression has been implicated in breast, prostate, and liver cancers and therefore is an attractive target for cancer therapy. To date, little about the kinetic properties of CARM1 is known. In this study, substrate specificity and the kinetic mechanism of the human enzyme were determined. Substrate specificity was examined by testing CARM1 activity with several histone H3-based peptides in a radiometric assay. Comparison of k_cat/K_M values reveals that methylation of H3R17 is preferred over that of H3R26. These effects are K_M-driven as k_cat values remain relatively constant for the peptides tested. Shortening the peptide at the C-terminus by five amino acid residues greatly reduced binding affinity, indicating distal residues may contribute to substrate binding. CARM1 appears to bind monomethylated peptides with an affinity similar to that of unmethylated peptides. Monitoring of the CARM1-dependent production of monomethylated and dimethylated peptides over time by self-assembled monolayer and matrix-assisted laser desorption ionization mass spectrometry revealed that methylation by CARM1 is distributive. Additionally, dead-end and product inhibition studies suggest CARM1 conforms to a random sequential kinetic mechanism. By defining the kinetic properties and mechanism of CARM1, these studies may aid in the development of small molecule CARM1 inhibitors.