文摘
Volatiles generated from lipoxygenase (LOX) normal and LOX deficient soybean (Glycine max)varieties with and without added lipase inhibited Aspergillus flavus mycelial growth and aflatoxinproduction. Soybean volatiles were analyzed using a solid phase microextraction (SPME) methodcombined with gas chromatography-mass spectrometry (GC-MS). Twenty-one compounds, including11 aldehydes, three alcohols, four ketones, one furan, one alkane, and one alkene were detected inthe LOX normal soybean line. However, only nine volatile compounds were observed in the LOXdeficient soybean variety. The antifungal aldehydes hexanal and (E)-2-hexenal were observed inboth LOX normal and LOX deficient lines and were detected at significantly higher amounts in soybeanhomogenate with added lipase. These aldehydes may be formed through alternate pathways, otherthan the LOX pathway, and may account for the inhibition of A. flavus growth observed. Other volatilesdetected, particularly the ketones and alcohols, may contribute to the antifungal activity observed inboth LOX normal and LOX deficient soybean lines. These results suggest that other factors, otherthan LOX activity, may better explain why soybeans are generally not as severely affected by A.flavus and aflatoxin contamination as other oilseed crops.Keywords: Aflatoxin; soybean; SPME; volatiles; Aspergillus flavus