Optimized Design and Synthesis of a Cell-Permeable Biarsenical Cyanine Probe for Imaging Tagged Cytosolic Bacterial Proteins

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• 作者：Na Fu ; Yijia Xiong ; Thomas C. Squier
• 刊名：Bioconjugate Chemistry
• 出版年：2013
• 出版时间：February 20, 2013
• 年：2013
• 卷：24
• 期：2
• 页码：251-259
• 全文大小：360K
• 年卷期：v.24,no.2(February 20, 2013)
• ISSN：1520-4812

文摘

To optimize cellular delivery and specific labeling of tagged cytosolic proteins by biarsenical fluorescent probes built around a cyanine dye (Cy3) scaffold, we have systematically varied the polarity of the N-alkyl chain (i.e., 4鈥? methylene groups appended by a sulfonate or methoxy ester moiety) and arsenic capping reagent (ethanedithiol versus benzenedithiol). Optimal live-cell labeling and visualization of tagged cytosolic proteins is reported using an ethanedithiol capping reagent with the uncharged methoxy ester functionalized N-alkyl chains. These measurements demonstrate the general utility of this new class of photostable and highly fluorescent biarsenical probes based on the cyanine dye scaffold for in vivo labeling of tagged cellular proteins for live cell imaging measurements of protein dynamics.