Generation of Polypeptide-Templated Gold Nanoparticles using Ionizing Radiation

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• 作者：Candace Rae Walker ; Karthik Pushpavanam ; Divya Geetha Nair ; Thrimoorthy Potta ; Caesario Sutiyoso ; Vikram D. Kodibagkar ; Stephen Sapareto ; John Chang ; Kaushal Rege
• 刊名：Langmuir
• 出版年：2013
• 出版时间：August 13, 2013
• 年：2013
• 卷：29
• 期：32
• 页码：10166-10173
• 全文大小：434K
• 年卷期：v.29,no.32(August 13, 2013)
• ISSN：1520-5827

文摘

Ionizing radiation, including 纬 rays and X-rays, are high-energy electromagnetic radiation with diverse applications in nuclear energy, astrophysics, and medicine. In this work, we describe the use of ionizing radiation and cysteine-containing elastin-like polypeptides (C_nELPs, where n = 2 or 12 cysteines in the polypeptide sequence) for the generation of gold nanoparticles. In the presence of C_nELPs, ionizing radiation doses higher than 175 Gy resulted in the formation of maroon-colored gold nanoparticle dispersions, with maximal absorbance at 520 nm, from colorless metal salts. Visible color changes were not observed in any of the control systems, indicating that ionizing radiation, gold salt solution, and C_nELPs were all required for nanoparticle formation. The hydrodynamic diameters of nanoparticles, determined using dynamic light scattering, were in the range of 80鈥?50 nm, while TEM imaging indicated the formation of gold cores 10鈥?0 nm in diameter. Interestingly, C₂ELPs formed 1鈥? nm diameter gold nanoparticles in the absence of radiation. Our results describe a facile method of nanoparticle formation in which nanoparticle size can be tailored based on radiation dose and C_nELP type. Further improvements in these polypeptide-based systems can lead to colorimetric detection of ionizing radiation in a variety of applications.