Origin of the High-Frequency Resonances in 1H NMR Spectra of Muscle Tissue: An in Vitro Slow Magic-Angle Spinning Study

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• 作者：Hanne Christine Bertram ; Hans J//pubs.acs.org/images/gifchars/oslash.gif" border="0">rgen Jakobsen ; and Ole Bæ ; kgaard Nielsen
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：April 20, 2005
• 年：2005
• 卷：53
• 期：8
• 页码：3229 - 3234
• 全文大小：94K
• 年卷期：v.53,no.8(April 20, 2005)
• ISSN：1520-5118

文摘

High-resolution slow magic-angle spinning (150 Hz) ¹H PASS NMR spectroscopy is performed onintact excised rat m. tibialis anterior. Untreated muscles and muscles in vitro incubated in Krebs-Ringers buffer based on deuterium oxide are investigated. In the high-frequency region of the ¹HNMR spectra, resonances from H4 (~7.1-7.2 ppm) and H2 (~8.2-8.5 ppm) in histidine are observed.In addition, a resonance appears at 6.7 ppm for the untreated muscles. However, this resonance isabsent in muscles following incubation in deuterium oxide. On the basis of its behavior in deuteriumoxide combined with supplementary measurements for creatine solutions, the 6.7 ppm resonance isascribed to the amino protons in creatine. Moreover, the present study demonstrates that theobservation of the 6.7 ppm resonance depends on pH, which explains earlier reports stating itsoccasional appearance. Finally, measurements on solutions of ATP/AMP and histidine indicate thatboth ATP/AMP and histidine contribute to the resonances at ~8.2-8.5 ppm in the ¹H NMR spectraof muscle tissue.Keywords: Creatine; histidine; ATP; post-mortem muscle; pH; MAS; high-resolution ¹H NMRspectroscopy; PASS