Integrated System for Rapid PCR-Based DNA Analysis in Microfluidic Devices

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• 作者：Julia Khandurina ; Timothy E. McKnight ; Stephen C. Jacobson ; Larry C. Waters ; Robert S. Foote ; and J. Michael Ramsey
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：July 1, 2000
• 年：2000
• 卷：72
• 期：13
• 页码：2995 - 3000
• 全文大小：123K
• 年卷期：v.72,no.13(July 1, 2000)
• ISSN：1520-6882

文摘

An integrated system for rapid PCR-based analysis on amicrochip has been demonstrated. The system couples acompact thermal cycling assembly based on dual Peltierthermoelectric elements with a microchip gel electrophoresis platform. This configuration allows fast (~1 min/cycle) and efficient DNA amplification on-chip followedby electrophoretic sizing and detection on the same chip.An on-chip DNA concentration technique has been incorporated into the system to further reduce analysis timeby decreasing the number of thermal cycles required. Theconcentration injection scheme enables detection of PCRproducts after performing as few as 10 thermal cycles,with a total analysis time of less than 20 min. The startingtemplate copy number was less than 15 per injectionvolume.