Fast Analysis of Nicotine in Tobacco Using Double-Shot Pyrolysis-Gas Chromatography-Mass Spectrometry
详细信息 查看全文
- 作者：Jae Gon Lee ; Chang Gook Lee ; Jae Jin Kwag ; Moon Soo Rhee ; Alan J. Buglass ; Gae Ho Lee
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：February 21, 2007
- 年：2007
- 卷：55
- 期：4
- 页码：1097 - 1102
- 全文大小：95K
- 年卷期：v.55,no.4(February 21, 2007)
- ISSN：1520-5118
文摘
A simple, rapid, and sensitive assay of nicotine in tobacco has been developed using pyrolysis-gaschromatography-mass spectrometry (DSP-GC-MS). The optimum pyrolyzer (desorption) conditions,using Korean tobacco grade B1O (0.4 mg) and n-heptadecane as an internal standard, were foundto be heating from 50 to 300 
C at a rate of 60 C/min. Replicate determinations (n = 5), on thesame tobacco and using n-heptadecane as the internal standard, resulted in a nicotine content of1.96%, with a relative standard deviation (RSD) of 1.9%. This result was in good agreement withthose from established methods: the Cai ether extraction method, a chloroform extraction method,and the CORESTA recommended method. However, the DSP method requires less than half thetime of the solvent extraction methods, requires less sample, is almost solvent-free, and is less labor-intensive. The DSP method was used to determine the nicotine content of eight flue-cured tobaccosfrom Brazil, China, Korea, and the United States, which were found to have contents ranging from1.21 to 2.19%.
C at a rate of 60 C/min. Replicate determinations (n = 5), on thesame tobacco and using n-heptadecane as the internal standard, resulted in a nicotine content of1.96%, with a relative standard deviation (RSD) of 1.9%. This result was in good agreement withthose from established methods: the Cai ether extraction method, a chloroform extraction method,and the CORESTA recommended method. However, the DSP method requires less than half thetime of the solvent extraction methods, requires less sample, is almost solvent-free, and is less labor-intensive. The DSP method was used to determine the nicotine content of eight flue-cured tobaccosfrom Brazil, China, Korea, and the United States, which were found to have contents ranging from1.21 to 2.19%.