Bifunctional Nanotube Scaffolds for Diverse Ligands Are Purified Simply from Escherichia coli Strains Coexpressing Two Functionalized Flagellar Genes
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- 作者：Richard D. Woods ; Noriko Takahashi ; Akhmed Aslam ; Richard J. Pleass ; Shin-Ichi Aizawa ; R. Elizabeth Sockett
- 刊名：Nano Letters
- 出版年：2007
- 出版时间：June 2007
- 年：2007
- 卷：7
- 期：6
- 页码：1809 - 1816
- 全文大小：461K
- 年卷期：v.7,no.6(June 2007)
- ISSN：1530-6992
文摘
We functionalized Escherichia coli FliC flagellin proteins to form tailored nanotubes binding single types or pairs of ligands, including divalentcations, fluorescent antibodies, or biotin-avidin-linked moieties such as ferritins. The ratio of each tag in bifunctionalized flagella could betoggled extending their sophistication as nanoscaffolds. Tobacco Etch Virus (TEV) protease site-containing FliCs were cleaved by the cognateprotease without filament disintegration, potentiating their use as removable nanolithography masks to deposit attached ligands by proteasecleavage.