Anti-hyaluronidase Activity in Vitro and Amelioration of Mouse Experimental Dermatitis by Tomato Saponin, Esculeoside A

详细信息    查看全文

• 作者：Jian-Rong Zhou ; Yurina Kanda ; Anna Tanaka ; Hideyuki Manabe ; Toshihiro Nohara ; Kazumi Yokomizo
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：January 20, 2016
• 年：2016
• 卷：64
• 期：2
• 页码：403-408
• 全文大小：401K
• ISSN：1520-5118

文摘

The increasing incidence of atopic dermatitis during recent decades has prompted the development of safe and effective agents for prevention of atopic diseases. Esculeoside A, a glycoside of spirosolane type, is identified as a major component in ripe tomato fruits. The present study investigated the effects of esculeoside A and its aglycon esculeogenin A on hyaluronidase activity in vitro and antiallergy in experimental dermatitis mice. Esculeogenin A/esculeoside A (esculeogenin A equivalent) with an IC₅₀ of about 2 μM/9 μM dose-dependently inhibited hyaluronidase activity measured by a modified Morgan–Elson method. Oral treatment with esculeoside A 10 mg/kg of experimental dermatitis mice for 4 weeks significantly decreased the skin clinical score to 2.5 without any detectable side effects compared with 6.75 of the control. The scratching frequency of esculeoside A 100 mg/kg application was decreased significantly as 107.5 times compared with 296.67 times of the control. Thus, the present study showed that esculeoside A/esculeogenin A significantly blocks hyaluronidase activity in vitro and that esculeoside A ameliorates mouse experimental dermatitis.