Synergistic Potentials of Coffee on Injured Pancreatic Islets and Insulin Action via KATP Channel Blocking in Zebrafish
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- 作者：Youn Hee Nam ; Bin Na Hong ; Isabel Rodriguez ; Min Gun Ji ; Keonwoo Kim ; Ung-Jin Kim ; Tong Ho Kang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：June 17, 2015
- 年：2015
- 卷：63
- 期：23
- 页码：5612-5621
- 全文大小：547K
- ISSN：1520-5118
文摘
Pancreatic islets (PIs) are damaged under diabetic conditions, resulting in decreased PI size. This study examined the regenerative effects of coffee and its components (caffeine, CFI; trigonelline, TRG; chlorogenic acid, CGA) on zebrafish larval PIs and 尾-cells damaged by administration of alloxan (AX). In addition, the influence of coffee and its active components on KATP channels was investigated using diazoxide (DZ) as a KATP channel activator. PI size and fluorescence intensity were significantly increased in the coffee-treated group relative to the no-treatment group (P < 0.0001). In addition, coffee exerted significant regenerative effects on pancreatic 尾-cells (p = 0.006). Treatment with TRG and CGA rescued PI damage, and the combination of TRG/CGA had a synergistic effect. In conclusion, the results indicate that coffee has beneficial effects on AX-damaged PIs and may also be useful as a blocker of pancreatic 尾-cell K+ channels.