Dicationic Lipophosphoramidates as DNA Carriers

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• 作者：Mathieu Mé ; vel ; Tristan Montier ; Franç ; ois Lamarche ; Pascal Delé ; pine ; Tony Le Gall ; Jean-Jacques Yaouanc ; Paul-Alain Jaffrè ; s ; Dominique Cartier ; Pierre Lehn ; Jean-Claude Cl&ea
• 刊名：Bioconjugate Chemistry
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：18
• 期：5
• 页码：1604 - 1611
• 全文大小：477K
• 年卷期：v.18,no.5(September 2007)
• ISSN：1520-4812

文摘

Lipophosphoramidates with two different permanent cations as polar heads were synthesized and evaluated fortheir gene transfer activity. Physicochemical measurements (particle size, zeta potentials) and gel retardationassays were also performed. In vitro biological evaluation was conducted with A542 and HeLa cell lines, andcytotoxicity determined by a chemiluminescent assay. The set of results indicates that, on the whole, dicationiclipophosphoramidates constitute an interesting alternative to their monocationic analogues.