Toward Full Peptide Sequence Coverage by Dual Fragmentation Combining Electron-Transfer and Higher-Energy Collision Dissociation Tandem Mass Spectrometry

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• 作者：Christian K. Frese ; A. F. Maarten Altelaar ; Henk van den Toorn ; Dirk Nolting ; Jens Griep-Raming ; Albert J. R. Heck ; Shabaz Mohammed
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：November 20, 2012
• 年：2012
• 卷：84
• 期：22
• 页码：9668-9673
• 全文大小：293K
• 年卷期：v.84,no.22(November 20, 2012)
• ISSN：1520-6882

文摘

Increasing peptide sequence coverage by tandem mass spectrometry improves confidence in database search-based peptide identification and facilitates mapping of post-translational modifications and de novo sequencing. Inducing 2-fold fragmentation by combining electron-transfer and higher-energy collision dissociation (EThcD) generates dual fragment ion series and facilitates extensive peptide backbone fragmentation. After an initial electron-transfer dissociation step, all ions including the unreacted precursor ions are subjected to collision induced dissociation which yields b/y- and c/z-type fragment ions in a single spectrum. This new fragmentation scheme provides richer spectra and substantially increases the peptide sequence coverage and confidence in peptide identification.