Reversible Holographic Patterns on Azopolymers for Guiding Cell Adhesion and Orientation

详细信息    查看全文

• 作者：Carmela Rianna ; Alejandro Calabuig ; Maurizio Ventre ; Silvia Cavalli ; Vito Pagliarulo ; Simonetta Grilli ; Pietro Ferraro ; Paolo A. Netti
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：August 12, 2015
• 年：2015
• 卷：7
• 期：31
• 页码：16984-16991
• 全文大小：431K
• ISSN：1944-8252

文摘

Topography of material surfaces is known to influence cell behavior at different levels: from adhesion up to differentiation. Different micro- and nanopatterning techniques have been employed to create patterned surfaces to investigate various aspects of cell behavior, most notably cellular mechanotransduction. Nevertheless, conventional techniques, once implemented on a specific substrate, fail in allowing dynamic changes of the topographic features. Here we investigated the response of NIH-3T3 cells to reversible topographic signals encoded on light-responsive azopolymer films. Switchable patterns were fabricated by means of a well-established holographic setup. Surface relief gratings were realized with Lloyd鈥檚 mirror system and erased with circularly polarized or incoherent light. Cell cytoskeleton organization and focal adhesion assembly proved to be very sensitive to the underlying topographic signal. Thereafter, pattern reversibility was tested in air and wet environment by using temperature or light as a trigger. Additionally, pattern modification was dynamically performed on substrates with living cells. This study paves the way toward an in situ and real-time investigation of the material鈥揷ytoskeleton crosstalk caused by the intrinsic properties of azopolymers.