Osteoblast Adhesion on Poly(L-lactic Acid)/Polystyrene Demixed Thin Film Blends: Effect of Nanotopography, Surface Chemistry, and Wettability
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- 作者：Jung Yul Lim ; Joshua C. Hansen ; Christopher A. Siedlecki ; Robert W. Hengstebeck ; Juan Cheng ; Nicholas Winograd ; and Henry J. Donahue
- 刊名：Biomacromolecules
- 出版年：2005
- 出版时间：November 2005
- 年：2005
- 卷：6
- 期：6
- 页码：3319 - 3327
- 全文大小：457K
- 年卷期：v.6,no.6(November 2005)
- ISSN：1526-4602
文摘
Biomaterial surface characteristics are critical cues that regulate cell function. We produced a novel seriesof poly(L-lactic acid) (PLLA) and polystyrene demixed nanotopographic films to provide nonbiologicalcell-stimulating cues. The increase in PLLA weight fraction (
) in blend solutions resulted in topographychanges in spin-cast films from pit-dominant to island-dominant morphologies having nanoscale depth orheight (3-29 nm). Lower molecular weight PLLA segregated to the top surface of demixed films, as observedby X-ray photoelectron spectroscopy and secondary ion mass spectroscopy (SIMS). For 
0.5, the topmostfilm layer was predominantly filled with PLLA (>96% by SIMS at 20-Å depth). Nanotextured substratastimulated osteoblastic cell adhesion to a greater degree than did flat PLLA ( = 1), and this effect wasmore pronounced for nanoisland ( = 0.7 and 0.9) relative to nanopit topographies ( = 0.5). Demixedfilms having relatively lower water contact angles generally enhanced cell adhesion and spreading. Ourresults reveal that cell adhesion is affected by surface chemistry, topography, and wettability simultaneouslyand that nanotextured surfaces may be utilized in regulating cell adhesion.
) in blend solutions resulted in topographychanges in spin-cast films from pit-dominant to island-dominant morphologies having nanoscale depth orheight (3-29 nm). Lower molecular weight PLLA segregated to the top surface of demixed films, as observedby X-ray photoelectron spectroscopy and secondary ion mass spectroscopy (SIMS). For 0.5, the topmostfilm layer was predominantly filled with PLLA (>96% by SIMS at 20-Å depth). Nanotextured substratastimulated osteoblastic cell adhesion to a greater degree than did flat PLLA ( = 1), and this effect wasmore pronounced for nanoisland ( = 0.7 and 0.9) relative to nanopit topographies ( = 0.5). Demixedfilms having relatively lower water contact angles generally enhanced cell adhesion and spreading. Ourresults reveal that cell adhesion is affected by surface chemistry, topography, and wettability simultaneouslyand that nanotextured surfaces may be utilized in regulating cell adhesion.