Apple Procyanidin Oligomers Absorption in Rats after Oral Administration: Analysis of Procyanidins in Plasma Using the Porter Method and High-Performance Liquid Chromatography/Tandem Mass Spectrometry

详细信息    查看全文

• 作者：Toshihiko Shoji ; Saeko Masumoto ; Nina Moriichi ; Hiroshi Akiyama ; Tomomasa Kanda ; Yasuyuki Ohtake ; and Yukihiro Goda
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：February 8, 2006
• 年：2006
• 卷：54
• 期：3
• 页码：884 - 892
• 全文大小：278K
• 年卷期：v.54,no.3(February 8, 2006)
• ISSN：1520-5118

文摘

In this study, we investigated the absorption of apple procyanidins, namely, apple condensed tannins(ACTs), in rats using the Porter method and high-performance liquid chromatography/tandem massspectrometry. The apple procyanidin concentrations in the rat plasma reached a maximum 2 h afteradministration and decreased thereafter. To investigate the limits of the absorption of appleprocyanidins in the polymerization degree, we administered the procyanidin oligomer fraction, whichwas separated from ACT using normal-phase chromatography according to the degree of polymerization. Procyanidins from each dimer to pentamer group were detected in the plasma by the Portermethod. Moreover, by the study using reconstituted procyanidins, polymeric procyanidins influencedthe absorption of procyanidin oligomers. These results suggest that ACTs are absorbed and directlyinvolved in physiological functions in the rats.Keywords: Absorption; apple procyanidins; HPLC/MS/MS; procyanidin dimers; procyanidin trimers