Photochemical Ligation of DNA Conjugates through Anthracene Cyclodimer Formation and Its Fidelity to the Template Sequences

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• 作者：Toshihiro Ihara ; Tomohiro Fujii ; Motoko Mukae ; Yusuke Kitamura ; and Akinori Jyo
• 刊名：Journal of the American Chemical Society
• 出版年：2004
• 出版时间：July 28, 2004
• 年：2004
• 卷：126
• 期：29
• 页码：8880 - 8881
• 全文大小：54K
• 年卷期：v.126,no.29(July 28, 2004)
• ISSN：1520-5126

文摘

Anthracene readily forms photoadducts, anthracene dimers, and this photodimerization reaction has been well characterized. In general, however, the reaction requires close proximity and certain spatial alignment of both reaction partners. DNA could provide an ideal scaffold for accelerating the photocyclic addition. We synthesized a number of anthracene-DNA conjugates. The sequences of the conjugates, 5'AntODNn and 3'AntODNn (the length of methylene linkers: n = 3 or 6), were designed to bind adjacent sequences of the template with the anthracene units directed such that they stacked with each other. The conjugates were only dimerized in the presence of the template by light irradiation. The efficiency was affected by one-base displacement in the template sequence.