Microstructure of Poly(γ-glutamic acid) Produced by Bacillus subtilis Consisting of Clusters of D- and L-Glutamic Acid Repeating Units

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• 作者：Fei Wang ; Masaji Ishiguro ; Mai Mutsukado ; Ken-ichi Fujita ; Toshio Tanaka
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：June 11, 2008
• 年：2008
• 卷：56
• 期：11
• 页码：4225 - 4228
• 全文大小：293K
• 年卷期：v.56,no.11(June 11, 2008)
• ISSN：1520-5118

文摘

Poly(γ-glutamic acid) (PGA) produced by a strain of Bacillus subtilis was partially hydrolyzed into various oligopeptides so that the dipeptide fraction was isolated by the preparative thin-layer chromatography. HPLC analysis was applied to the detection of each of the four stereoisomers in this fraction using chemically synthesized authentic samples. The fraction consisted of N-γ-D-glutamyl-D-glutamic acid, N-γ-L-glutamyl-L-glutamic acid, N-γ-D-glutamyl-L-glutamic acid, and N-γ-L-glutamyl-D-glutamic acid at a ratio of 5.9:6.0:1.0:1.0. On the basis of this result, a model was proposed for the microstructure of the bacterial PGA, in which D- and L-glutamic acid repeating units are alternately linked in a single chain of the molecule.