The Anti-atherosclerotic Dipeptide, Trp-His, Reduces Intestinal Inflammation through the Blockade of L-Type Ca2+ Channels

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• 作者：Yutaro Kobayashi ; Jennifer Kovacs-Nolan ; Toshiro Matsui ; Yoshinori Mine
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 8, 2015
• 年：2015
• 卷：63
• 期：26
• 页码：6041-6050
• 全文大小：472K
• ISSN：1520-5118

文摘

Trp-His, the anti-atherosclerotic dipeptide, exerted an antiproliferative effect on vascular smooth muscle cells by L-type Ca²⁺ channel blocker-like effect. The beneficial potential by the blockade of Ca²⁺ channels on chronic intestinal inflammation, including inflammatory bowel disease (IBD), is unclear. Trp-His (100 or 250 mg/kg body weight/day) was administered for 14 days to BALB/c mice, and 5% dextran sodium sulfate (DSS) was administered to induce colitis in the last 7 days. Trp-His reduced DSS-induced typical colitis symptoms and cytokine expression in the colon. Trp-His inhibited interleukin (IL)-8 secretion in tumor necrosis factor (TNF)-伪-stimulated HT-29 cells. The inhibitory effect of Trp-His, as well as that of Ca²⁺ channel blockers, was impaired by the presence of Ca²⁺ channel agonist Bay K 8644. The TNF-伪-induced activation of mitogen-activated protein kinases (MAPKs) and I魏B伪 were decreased by Trp-His. These results indicated that the anti-inflammatory effect of Trp-His may be involved in the blockade of L-type Ca²⁺ channels.