Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET

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• 作者：Tara Sabir ; Gunnar F. Schr枚der ; Anita Toulmin ; Peter McGlynn ; Steven W. Magennis
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：February 9, 2011
• 年：2011
• 卷：133
• 期：5
• 页码：1188-1191
• 全文大小：747K
• 年卷期：v.133,no.5(February 9, 2011)
• ISSN：1520-5126

文摘

Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA鈭抪rotein interaction studies.