Multicolor Fluorescence Imaging of Traumatic Brain Injury in a Cryolesion Mouse Model
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- 作者：Bryan A. Smith ; Bang-Wen Xie ; Ermond R. van Beek ; Ivo Que ; Vicky Blankevoort ; Shuzhang Xiao ; Erin L. Cole ; Mathias Hoehn ; Eric L. Kaijzel ; Clemens W. G. M. L枚wik ; Bradley D. Smith
- 刊名：ACS Chemical Neuroscience
- 出版年：2012
- 出版时间：July 18, 2012
- 年：2012
- 卷：3
- 期：7
- 页码：530-537
- 全文大小：370K
- 年卷期：v.3,no.7(July 18, 2012)
- ISSN：1948-7193
文摘
Traumatic brain injury is characterized by initial tissue damage, which then can lead to secondary processes such as cell death and blood-brain-barrier disruption. Clinical and preclinical studies of traumatic brain injury typically employ anatomical imaging techniques and there is a need for new molecular imaging methods that provide complementary biochemical information. Here, we assess the ability of a targeted, near-infrared fluorescent probe, named PSS-794, to detect cell death in a brain cryolesion mouse model that replicates certain features of traumatic brain injury. In short, the model involves brief contact of a cold rod to the head of a living, anesthetized mouse. Using noninvasive whole-body fluorescence imaging, PSS-794 permitted visualization of the cryolesion in the living animal. Ex vivo imaging and histological analysis confirmed PSS-794 localization to site of brain cell death. The nontargeted, deep-red Tracer-653 was validated as a tracer dye for monitoring blood-brain-barrier disruption, and a binary mixture of PSS-794 and Tracer-653 was employed for multicolor imaging of cell death and blood-brain-barrier permeability in a single animal. The imaging data indicates that at 3 days after brain cryoinjury the amount of cell death had decreased significantly, but the integrity of the blood-brain-barrier was still impaired; at 7 days, the blood-brain-barrier was still three times more permeable than before cryoinjury.