Synchrotron Micro-XRF and Micro-XANES Confirmation of the Uptake and Translocation of TiO2 Nanoparticles in Cucumber (Cucumis sativus) Plants

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• 作者：Alia D. Servin ; Hiram Castillo-Michel ; Jose A. Hernandez-Viezcas ; Baltazar Corral Diaz ; Jose R. Peralta-Videa ; Jorge L. Gardea-Torresdey
• 刊名：Environmental Science & Technology (ES&T)
• 出版年：2012
• 出版时间：July 17, 2012
• 年：2012
• 卷：46
• 期：14
• 页码：7637-7643
• 全文大小：483K
• 年卷期：v.46,no.14(July 17, 2012)
• ISSN：1520-5851

文摘

Advances in nanotechnology have raised concerns about possible effects of engineered nanomaterials (ENMs) in the environment, especially in terrestrial plants. In this research, the impacts of TiO₂ nanoparticles (NPs) were evaluated in hydroponically grown cucumber (Cucumis sativus) plants. Seven day old seedlings were treated with TiO₂ NPs at concentrations varying from 0 to 4000 mg L^鈥?. At harvest, the size of roots and shoots were measured. In addition, micro X- ray fluorescence (micro-XRF) and micro X-ray absorption spectroscopy (micro-XAS), respectively, were used to track the presence and chemical speciation of Ti within plant tissues. Results showed that at all concentrations, TiO₂ significantly increased root length (average >300%). By using micro-XRF it was found that Ti was transported from the roots to the leaf trichomes, suggesting that trichomes are possible sink or excretory system for the Ti. The micro-XANES spectra showed that the absorbed Ti was present as TiO₂ within the cucumber tissues, demonstrating that the TiO₂ NPs were not biotransformed.