Synthesis of Nucleoside Di- and Triphosphates and Dinucleoside Polyphosphates with cycloSal-Nucleotides
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- 作者：Svenja Warnecke ; Chris Meier
- 刊名：Journal of Organic Chemistry
- 出版年：2009
- 出版时间：April 17, 2009
- 年：2009
- 卷：74
- 期：8
- 页码：3024-3030
- 全文大小：231K
- 年卷期：v.74,no.8(April 17, 2009)
- ISSN：1520-6904
文摘
A new and efficient method for the synthesis of nucleoside di- and triphosphates as well as dinucleoside polyphosphates (NpnN′) is described. 5-Acceptor-substituted (5-nitro and 5-chloro) cycloSal-nucleotides are used as starting material that were reacted with a variety of phosphate nucleophiles as pyrophosphate or nucleotides to the corresponding products in short times and very good yields. After consumption of the starting cycloSal-phosphate triester, first the protecting groups were cleaved and finally the products were isolated after RP-column chromatography. Examples are shown for all five pyrimidine and purine bases found in natural nucleosides as well as one non-natural pyrimidine base to prove that the method can be applied generally.