DNA-Induced Aggregation of Zwitterionic Oligolamellar Liposome

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• 作者：Ching-Mao Wu ; Hsin-Lung Chen ; Willisa Liou ; Tsang-Lang Lin ; and U-Ser Jeng
• 刊名：Biomacromolecules
• 出版年：2004
• 出版时间：November 2004
• 年：2004
• 卷：5
• 期：6
• 页码：2324 - 2328
• 全文大小：309K
• 年卷期：v.5,no.6(November 2004)
• ISSN：1526-4602

文摘

Liposome consisting of a single zwitterionic lipid as the potential vector for gene therapy has been reportedrecently; however, whether polyanionic DNA can bind directly with zwitterionic lipid without the aid ofmultivalent salt still remains unresolved. In this study, we reveal the aggregation of zwitterionic oligolamellarliposomes composed of 1,2-di(cis-9-octadecenoyl)-sn-glycero-3-phosphocholine induced by DNA withoutthe presence of multivalent salt. Our results demonstrate that only a small fraction (<10%) of DNA canbind electrostatically with a portion of the liposomes. Such a low degree of binding, however, inducessignificant aggregation of these oligolamellar liposomes, yielding large multilamellar particles in which thenumber of hydrophilic/hydrophobic layer stacking becomes sufficiently large to yield multiple diffractionpeaks in the small-angle X-ray scattering profile. Addition of monovalent salt such as NaCl tends to disruptthe multilamellar structure.