New Luminescent Cyclometalated Iridium(III) Diimine Complexes as Biological Labeling Reagents
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- 作者：Kenneth Kam-Wing Lo ; Chi-Keung Chung ; Terence Kwok-Ming Lee ; Lok-Hei Lui ; Keith Hing-Kit Tsang ; and Nianyong Zhu
- 刊名：Inorganic Chemistry
- 出版年：2003
- 出版时间：October 20, 2003
- 年：2003
- 卷：42
- 期：21
- 页码：6886 - 6897
- 全文大小：166K
- 年卷期：v.42,no.21(October 20, 2003)
- ISSN：1520-510X
文摘
We report the synthesis, characterization, and photophysical and electrochemical properties of thirty luminescentcyclometalated iridium(III) diimine complexes [Ir(N-C)2(N-N)](PF6) (HN-C = 2-phenylpyridine, Hppy; 2-(4-methylphenyl)pyridine, Hmppy; 3-methyl-1-phenylpyrazole, Hmppz; 7,8-benzoquinoline, Hbzq; 2-phenylquinoline, Hpq; N-N =4-amino-2,2'-bipyridine, bpy-NH2; 4-isothiocyanato-2,2'-bipyridine, bpy-ITC; 4-iodoacetamido-2,2'-bipyridine, bpy-IAA; 5-amino-1,10-phenanthroline, phen-NH2; 5-isothiocyanato-1,10-phenanthroline, phen-ITC; 5-iodoacetamido-1,10-phenanthroline, phen-IAA). The X-ray crystal structure of [Ir(mppz)2(bpy-NH2)](PF6) has also been investigated.Upon irradiation, all the complexes display intense and long-lived luminescence under ambient conditions and in77-K glass. On the basis of the photophysical and electrochemical data, the emission of most of these complexesis assigned to an excited state of predominantly triplet metal-to-ligand charge-transfer (3MLCT) (d
(Ir) 
*(N-N))character. In some cases, triplet intraligand (3IL) ( *)(N-N or N-C-) excited states have also been identified.In view of the specific reactivity of the isothiocyanate and iodoacetamide moieties toward the primary amine andsulfhydryl groups, respectively, we have labeled various biological molecules with a selection of these luminescentiridium(III) complexes. The photophysical properties of the luminescent conjugates have been investigated. In addition,a heterogeneous assay for digoxin has also been designed on the basis of the recognition of biotinylated anti-digoxin by avidin labeled with one of the luminescent iridium(III) complexes.
(Ir) *(N-N))character. In some cases, triplet intraligand (3IL) ( *)(N-N or N-C-) excited states have also been identified.In view of the specific reactivity of the isothiocyanate and iodoacetamide moieties toward the primary amine andsulfhydryl groups, respectively, we have labeled various biological molecules with a selection of these luminescentiridium(III) complexes. The photophysical properties of the luminescent conjugates have been investigated. In addition,a heterogeneous assay for digoxin has also been designed on the basis of the recognition of biotinylated anti-digoxin by avidin labeled with one of the luminescent iridium(III) complexes.